Day 1 :
Babylon University, Iraq
Keynote: Red Beet Juice and Urine System
Time : 09:15-10:00
Jaleel Kareem Ahmed has his expertise in evaluation in iron and steel industry. He has registered 3 patents in USA, UK and Iraq about using water in iron industry and wax for storage and transportation and using wax for carburizing of steel. He has used chlorophyll as gamma ray absorber to protect Iraqi children from cancer and mechanically red beet juice as scavenger for poisonous heavy metal ions and anticancer and detoxification of urea and uric acid from human body via urine system. In 2013, he was awarded with Scientific Medal from Iraqi Government. In 2014, he got qualified as a Member in Who is Who network. He has been serving as a Reviewer of Journal of Advances in Polymer Technology.
A case study is carried out on the urine of a man with 40 years old. Two samples of urine are taken from the urine system one after drinking concentrated red beet juice (mechanically extracted) and the second one without drinking. Using Ultraviolet-visible absorption spectra measurements was done, the results showed that with concentrated juice the absorption bands are shifted toward low energy due to the hydrogen bond formation by exchangeable proton from the juice (anthocyanin pigments) to the lone pair of electrons on the oxygen and nitrogen atoms in uric acid and urea and vice versa which shifted the n-p* absorption band to the lower energy, while dilution the above sample spectrum shows shifting to the higher energy, this is due to the low hydrogen bonding formation with uric acid and urea due to the low concentration of exchangeable proton upon dilution, as well as water is a good hydrogen bonding competitor. From ultraviolet-visible spectra we conclude that absorption band shifted to the lower energy with drinking concentrated juice and to the higher energy with diluted one which reflects the importance of concentration of the juice on hydrogen bonding formation and on the enhancing of detoxification of uric acid and urea from the blood, thus we recommended high concentration juice which can obtain from red beet (highest concentration of anthocyanin than other fruit). Dilution of pure urine sample does not affect its spectrum; this is because water is already present in urine in a good quantity comparing with uric acid and urea concentrations thus no effect from more water. Results shows that with drinking high concentrated red beet juice viscosity, electrical conductivity and refractive index of exit urine decreased, which enhances detoxification process. The viscosity of urine with juice lower than viscosity of pure water which is 1.00 cP at 20 oC, this make urine+juice easier to flow through urine system than water alone. The pP of urine after drinking the juice is increased; this is due to the capture of the proton of uric acid by anthocyanin which is less acidic then former. The increase of pP results in lessens the tension of the human. The density of the urine increases slightly due to the more hydrogen bonding formation with the anthocyanin results in reducing the volume of the unit weight of the sample.
Institute of Biological Chemistry-Academia Sinica, Taiwan
Keynote: Regulation of BIK ubiquitination determines life-death fate of cellular stress responses and anti-tumor activity
Time : 11:15-12:00
Ruey-Hwa Chen has received BS and MS degrees from National Taiwan University and PhD degree from Michigan State University. In 2006, she relocated to Institute of Biological Chemistry, Academia Sinica to be a Research Fellow and promoted to Distinguished Research Fellow in 2012. She was also an Associate Professor and Professor at National Taiwan University. She has served as Deputy Director of Institute of Biological Chemistry in 2011-2013. She has received several awards, including Outstanding Scholar Research Grant, National Science Council; Merit Research Award, National Science Council; Outstanding Award, TienTe Lee Biomedical Foundation; TBF Chair in Biotechnology; the 59th Academic Award, The Ministry of Education; Merit MOST Research Fellow Award and Taiwan Outstanding Women in Science, Wu Chien-Shiung Education Foundation. Her current research focuses on protein ubiquitination in tumorigenesis and tumor progression and protein ubiquitination in autophagy.
The BH3-only pro-apoptotic protein BIK is regulated by ubiquitin-proteasome system. However, the underlying mechanism of this regulation and its physiological functions remain elusive. Here, we identify a BIK ubiquitination/degradation mechanism mediated by ubiquitin ligase Cul5ASB11. Under ER stress, ASB11 is transcriptionally activated by IRE1/XBP1 axis of unfolded protein responses, which results in an enhancement of BIK ubiquitination and proteolysis. Conversely, genotoxic agents act through p53 to down-regulate IRE1 and ASB11, thereby stabilizing BIK. These opposite regulations of ASB11-medaited BIK ubiquitination participate in part to the cell adaptation to ER stress and DNA damage-induced apoptosis. Finally, IRE1 inhibitors stabilize the active form of BIK and increase its anti-tumor efficacy in triple negative breast cancers. Together, our study identifies a BIK ubiquitin ligase, uncovers the opposite regulations of this BIK ubiquitination by ER stress and DNA damage, and exploits the targeting of BIK ubiquitination pathway combined with active BIK for cancer therapy.
Tokai University, japan
Keynote: Automatic control of physiological state for future clinical treatment- Necessity of mathematical models
Time : 12:00-12:45
Tomohiko Utsuki has obtained his PhD in Health Science from Tokyo Medical and Dental University, Japan. He is currently an Associate Professor of Biomedical Engineering at Tokai University, Japan. His research interest is in automatic control of physiological state for future medical treatment; especially he has developed an automatic control system of brain temperature and applied it to patients for clinical hypothermia in the intensive care unit of Tokyo Medical and Dental University, Medical Hospital. Presently, he is constructing models of cerebral blood flow, intracranial pressure and brain temperature in consideration of their distribution in brain tissue in order to develop an automatic integrated control system of brain's physiological state for more advanced brain resuscitation treatment.
In operating room, emergency room and intensive care unit, medical staff have so heavy burden that they can't sometimes have enough time and attention for clinical treatment. Especially, many routine tasks, which demand advanced knowledge and skills to medical staff, are required for the management of physiological state pertinent to life-support. Thus, it is very useful to develop and apply automatic control systems of physiological state for alleviating such burden in clinical treatment. A few of systems have been developed and attempted to apply to patients in clinical treatment, such as automatic control systems of blood-sugar level, hemoglobin oxygen saturation in artery and concentration of carbon dioxide in alveoli. Notably, an automatic control system of brain temperature was developed and clinically applied. The designing and development of such system usually require a model of relevant physiological state as the controlled object in the simulation test of the system or as the element added in the system's controller, because heuristic system designing based on experiments on animals is not allowed ethically. On the stage of basic development, even if a system is designed by a method without using model, such as ‘Fictitious Reference Iterative Tuning’, a model of physiological state is necessary for verification of the system by simulation. In this presentation, various automatic control systems of physiological state are presented including each model used in the development. Moreover, a model of cerebral blood circulation and extracellular fluid migrations in cerebral tissue are explained, which are constructed as the elements of the integrative model of brain’s physiological state. This integrative model is required for developing an integrated control system, by which intracranial pressure, cerebral blood flow and brain temperature will be automatically controlled in consideration of the mutual effects for advanced brain resuscitation.